Efficacy of Various Decontamination Methods and Sterilization on Contaminated and Inoculated Diamond-Coated Burs
Abstract
The objective of this study was to evaluate the effectiveness of various decontamination methods and subsequent sterilization on contaminated and inoculated diamond-coated burs. Diamond-coated burs and extracted human molars were sterilized with a steam sterilizer. Enamel and dentin from the extracted teeth were abraded utilizing diamond-coated burs using a high-speed handpiece. The burs were subsequently inoculated with one of the following microorganisms: Enterococcus faecalis ATCC 19433, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442 or Geobacillus stearothermophilus ATCC 7953. Twenty-four hours after inoculation, the burs were subjected to various cleaning treatments, sterilized, and then cultured for bacterial contamination. The number of CFU/mL was determined per group. Except for the positive control group, no CFU/mL or growth was found for all treatment groups and for all bacterial types. In conclusion, the contaminated and inoculated diamond-coated burs tested in this study were successfully sterilized to eliminate the tested bacteria. The use of a cleaning stone with manual cleaning or an ultrasonic cleaner resulted in the least amount of remaining tooth debris on the diamond-coated bur heads.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 03, 2020
- Accession Number
- AD1105206
Entities
People
- Daniel Savett
- Kraig S. Vandewalle
- Michael Crabtree
- Nicole M. Wirth
- Wen Lien
Organizations
- 59th Medical Wing