Evaluation of the Biomeme Franklin Human Performance Factors: Final Report

Abstract

U.S. Army Combat Capabilities Development Command Chemical Biological Center (CCDC CBC) scientists completed an evaluation of the human performance factors related to the execution of COVID-19 testing using the SARS-CoV-2 Test Kit including (1) ease of use, (2) workflow development and assessment, and (3) time to results and sample throughput. The assay was found to require a fairly complex laboratory in order to safely process the samples due to the need for a biosafety cabinet to contain any SARS-CoV-2 containing aerosols. The workflow required nearly 3.5 h to complete 7 extractions, set up the seven RT-PCR reactions plus the two control RT-PCR reactions, run the RT-PCR on the Biomeme Franklin thermocycler, and interpret results. Therefore, throughput was estimated at 42 samples per 24 period on a single Franklin thermocycler.

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Document Details

Document Type
Technical Report
Publication Date
Aug 10, 2020
Accession Number
AD1105851

Entities

People

  • Bryan A. Rivers
  • Kimberly L. Berk
  • Patricia E. Buckley
  • Steven M. Blum

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biosafety
  • Covid-19
  • Extraction
  • Instruction Manuals
  • Instructions
  • Job Training
  • Materials
  • Mobile Application Software
  • Mobile Phones
  • Motor Skills
  • Pcr Testing
  • Polymerase Chain Reaction
  • Quality Control
  • Sars
  • Test And Evaluation
  • Thermal Cyclers
  • Virus Diseases

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