Effects of Ionizing Radiation (IR) on Myocardial DNA Methylation Profiles in Relation to Cardiomyopathy in a Nonhuman Primate Model
Abstract
Specific Aims were to determine 1: The effects of radiation exposure on the myocardial DNA methylome; 2: Relationships between myocardial methylation profiles and cardiomyopathy (myocardial fibrosis, heart structural and functional characteristics; and 3: Relationships between myocardial DNA methylation profiles and myocardial transcriptional profiles, the latter supported by another mechanism. Progress: Aim 1: We have completed the tissue distribution and processing, DNA isolation and characterization, and are finalizing plans for the DNA methylation and analysis work. Aim 2: Cardiac phenotyping completed. Aim 3: We are conducting analysis of the transcriptomes of these same tissues using RNAseq as well as Nanostring technologies, providing significant support for the planned methylation profiling. Our work on the myocardial gene expression profiles and other cardiac phenotyping required for final completion of a manuscript has been substantial and sets the stage for a significant body of work when combined with the proposed work.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2020
- Accession Number
- AD1106359
Entities
People
- Thomas C. Register
Organizations
- Wake Forest University