Prevention of Breast Cancer Skeletal Metastases with Parathyroid Hormone

Abstract

During this research period we have demonstrated that parathyroid hormone (PTH) started at the same time as intramammary implantation of murine 4T1-BGL breast cancer cells can decrease metastases to the skeleton but not to lung, liver or spleen. This is associated with changes in expression of metastasis genes in bone. We have further demonstrated that 4 weeks of pretreatment with PTH can decrease engraftment and growth of murine or human breast cancer cells injected into the intratibial bone marrow cavity. PTH also decreases the migration of breast cancer cells towards osteoblasts in a Transwell assay. These effects of PTH are mediated by decreasing expression of VCAM-1 in osteoblasts. Vcam1 mRNA and VCAM-1 protein are decreased in bones treated with PTH. Breast cancer cells engineered to overexpress VCAM-1increase migration towards osteoblasts, and this is blunted by PTH treatment.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2020
Accession Number
AD1106969

Entities

People

  • Joy Y. Wu

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Bone And Bones
  • Bone Diseases
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Diseases And Disorders
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Proteins

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology and Pathology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology