Regulatory Mechanisms of Radioresistance under Differential Levels of Ionizing Irradiation
Abstract
Unraveling mechanisms of cell survival under oxidative stress is important to protect human health and increase human performance in harsh environments. As an extremophile that can withstand unusually high levels of ionizing radiation, Deinococcus radiodurans hides a wealth of strategies to manage oxidative stresses that are not fully understood. The overarching goal of this project was to characterize the function and biological importance of our newly discovered RNA regulators, in the context of ionizing radiation and chemically induced oxidative stress. We proposed to profile some of the most active candidates to investigate new hypotheses that: (1) different mechanisms of oxidative stress management are triggered by different levels of radiation, and (2) that previously uncovered regulatory elements (i.e. 5'UTR) remain largely functional during oxidative stress to control transporters that are critical in initially boosting antioxidants while decreasing cellular toxins. The proposed work builds upon our recent discovery of 54 novel potential 5'UTR riboswitches that differentially regulate gene expression under relatively high (~15 kGy) and low (1 kGy) ionizing radiation and are largely found upstream of cellular transport genes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 12, 2020
- Accession Number
- AD1107175
Entities
People
- Lydia M Contreras
Organizations
- University of Texas at Austin