Single-molecule Bioelectronic Devices with Multi Sensing Capability
Abstract
In this project we developed strategies to assemble bioelectronic interfaces where biomolecules of interest were interfaced to carbon nanotubes (CNTs) with control down to the single-molecule level. We employed the CNTs as nanoelectrodes and transducers in sensing platforms, investigated the coupling between proteins and aptamers to CNTs, and organized these CNT biohybrids in nanoscale biosensing devices also with multiplexing capabilities. In particular we achieved the site-specific coupling of single proteins to individual CNTs with single-molecule control, and demonstrated the importance of bioengineering optimal protein attachment sites for direct protein nanotube communication at the single-protein level. Moreover, we demonstrated the assembly of both static and stimuli-responsive single-molecule heterostructures, where the distance and electronic coupling between an individual functional nanomoiety and a CNT are tuned via the use of DNA linkers. Finally, we fabricated reconfigurable and solution processable nanoscale realtime biosensing devices with multisensing capability based on CNTs functionalized with specific, and different, aptamer sequences employed as selective recognition elements for three different biomarkers indicative of stress and neuro-trauma conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 03, 2020
- Accession Number
- AD1108828
Entities
People
- Matteo Palma
Organizations
- Queen Mary University of London