Exosome Therapy for Stabilization of Extremity Injury

Abstract

Cellular therapies have tremendous potential for the successful treatment of major extremity wounds in the combat setting; however, the challenges associated with transplanting stem cells in the prolonged field care (PFC) environment are a critical barrier to progress in treating such injuries. Our goal is develop a new strategy utilizing extracellular vesicles (EVs) secreted by stem cells that can resolve many of these issues. Aim 1 of the project is to determine the optimal dosage and storage conditions of lyophilized extracellular vesicles (EVs) for enhancing cell survival in an in vitro model of muscle ischemia. Aim 2 will determine the impact of EV treatment on tissue preservation and recovery utilizing in vivo models of hindlimb ischemia-reperfusion injury. The proposed research, by advancing stem cell EV therapy, will therefore serve the public purpose by addressing the healthcare needs of not only active duty military personnel, their families, and veterans, but also civilians for whom ischemic injury is a major cause of morbidity and mortality.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2020
Accession Number
AD1109478

Entities

People

  • Mark W Hamrick

Organizations

  • Augusta University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Active Duty
  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Connective Tissue
  • Covid-19
  • Energy Production
  • Environment
  • Freeze Drying
  • Game Theory
  • Hydrogen Sulfides
  • Medical Personnel
  • Military Personnel
  • Muscle Cells
  • Myoblasts
  • Stem Cells
  • Students
  • Therapy
  • Tissues
  • Vascular Diseases

Fields of Study

  • Medicine

Readers

  • Oncology (Cancer Research).
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology