Compressing time and space for an in situ dermal graft printing paradigm
Abstract
Currently, no studies have investigated excised wound beds as a supportive matrix for a well-defined population of stem cells cultured directly within the wound bed niche. To capitalize on the idea of a wound bed-stem cell synergy, the discovery science phase of work in this proposal will rest on the premise that the native wound microenvironment will be the optimal bioreactor for a stem cell-based dermal graft model. Over the past year (Year 1) of the project, the PI team has completed the initial electrohydrodynamic printing process development phase towards fabricating high resolution 3D biological templates for stem cell-wound bed culture. The systematic design of experiments carried out in the current reporting period yielded significant results, including the quantitative effect of deposition time and collector temperature on fiber accumulation geometry, the effect of collector temperature on the interaction between two adjacent fibers, and that, to print 3D template structures, a toolpath optimization step is required to ensure straight, ordered fiber prints. For the next reporting report, systematic studies for the 3D template printing as a function of collector temperature will be analyzed. Stem cell -3D template interactions will be assessed for potential as a scalable in situ dermal graft model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2020
- Accession Number
- AD1110074
Entities
People
- Robert C. Chang
Organizations
- Stevens Institute of Technology