Molecular and Cellular Determinants of Malignant Transformation in Lung Squamous Cell Carcinoma

Abstract

During the second funding period we completed Major Tasks 1 and 2 and partially completed Major Task 3. The areas of interest were identified in 22 squamous cell lung cancer patients and isolated by LCM. Genomic DNA was isolated from these areas and whole exome sequencing was performed. The data has been analyzed for the progression-associated, premalignant- and malignant-specific mutations. The mutational data is being analyzed in the pathway context. Gene copy number alterations were identified by the analysis of the sequencing data. Multiplex immunofluorescence staining to determine the spectrum of infiltrating immune cells in tumor and premalignant microenvironment is ongoing.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2019
Accession Number
AD1110691

Entities

People

  • Steven M. Dubinett

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Biological Staining And Labeling
  • Biomedical Research
  • Cancer
  • Cancer Screening
  • Cells
  • Data Analysis
  • Department Of Defense
  • Genetic Variation
  • Information Operations
  • Law
  • Lung Cancer
  • Lymphocytes
  • Medical Personnel
  • Mutations
  • Neoplasms
  • Professional Development
  • Technology Transfer

Fields of Study

  • Biology

Readers

  • Oncology
  • Oncology and Biomarker-Based Cancer Detection.