Sub-Diffraction Temperature Mapping of Protein Interconversions
Abstract
The ability to spatially and temporally map nanoscale environments would be highly transformative for revealing dynamic biophysical phenomena. As investigations of time-dependent processes demand broad time observation windows previously unreachable by fluorescence super-resolution, a nonbleaching strategy is needed. We developed precision optics to suppress errors required for subdiffraction imaging. We scaled this method to unlimited numbers of scatterers. With a nonbleaching subdiffraction approach capable of broad timescales, we followed the temporal evolution of actin materials with subdiffraction resolution over broad time periods.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 15, 2020
- Accession Number
- AD1112107
Entities
People
- Somin Lee
Organizations
- University of Michigan