Fabrication of Autonomously Constructed Engineered Three-dimensional Shapes (FACETS)
Abstract
Major Goals: The project consists of two phases. In Phase 1, the objective was to explore design approaches for genetically programming a single cell line to give rise to a multicellular system with a predetermined three-dimensional form. In Phase 2 was to design and build tunable control of three-dimensional form in a clonal cell population, with differentiated surfaces. Phase 1 objectives included modeling and simulation, circuit design and construction, experiments, and a demo of three-dimensional sizes and shapes that are stable for at least 1 week under optimal growth/differentiation. Phase 1 simulation objectives included development of theoretical models that bridge developmental biology and synthetic biology objectives to achieve programmed development of morphology in an expanding clonal cell population. Potential issues in design were identified (see discussion below) and simulations were adjusted based on experimental data. Phase 1 objectives also included circuit design, construction, and experiments for interior / exterior circuits and other morphology circuits. Phase 2 objectives included modeling and simulation, circuit design and construction, experiments, and a demonstration of programmed forms that are stable in size and shape over a minimum period of 1 week under optimal growth/differentiation conditions. Simulations and modeling objectives included creating of a shape compiler for fast and accurate modeling. Phase 2 theoretical modeling objects were to predict points and potential mechanisms of control of morphology development. Simulations were Adjusted based on experimental data to refine predictions, including new objectives for cell-sorting based shape and image analysis. Phase 2 circuit objectives included design, construction, and experiments of both shape selection and facet selection circuits.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 15, 2019
- Accession Number
- AD1112403
Entities
People
- Ron Weiss
Organizations
- Massachusetts Institute of Technology