A New Paradigm for Radiation-Induced Persistent Cellular Stress and Genomic Instability in Lung Carcinogenesis

Abstract

We study the cellular stress responses to sublethal doses of 1 or 2 Gy X Rays that persist for up to a week in cultures of a human immortalized normal bronchial epithelial cell line, HBEC3-KT and evaluate their effect on cell transformation. The goal of this project was to evaluate whether altered chromatin ubiquitination is downstream of the p38MAPK activity, a key effector of the stress responses leading to persistent genomic instability and cellular transformation. Although several technical difficulties prevented us from evaluating therole of chromatin ubiquitination in this response, we identified mild replication stress and the activity of the transcription factor FOXM1b as two determinant factors in promoting radiation-induced genomic instability and cell transformation in HBEC3-KT cells.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2020
Accession Number
AD1113710

Entities

People

  • Erica Werner

Organizations

  • Emory University

Tags

DTIC Thesaurus Topics

  • Blood
  • Cancer
  • Cardiovascular Diseases
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Chromosome Aberrations
  • Culture Techniques
  • Epithelial Cells
  • Genetics
  • Lung Cancer
  • Oncology
  • Proteins
  • Proteomics
  • Stem Cells
  • United States

Readers

  • Molecular Biology and Genetics
  • Nuclear and Radiation Engineering.
  • Psychological Intervention/Treatment for Stress, Anxiety, PTSD, and Related Emotional and Cognitive Health Symptoms.