Calcium Signaling in Skeletal Muscle Atrophy: A Novel Role for the ERG1alpha K^+ Channel
Abstract
The ERG1A potassium channel is up-regulated in atrophic skeletal muscle and increases proteolysis when it is ectopically expressed in muscle. We have shown that, when it is expressed in cultured C2C12 myotubes, ERG1A increases the basal intracellular calcium concentration; however, the mechanism by which this occurs and the consequences of this are not known. We proposed to investigate the mechanism by which ERG1A increases intracellular calcium and the downstream effect of this on calpain enzyme-mediated proteolysis. To date, we have completed Major Task 2, determining that ERG1A does increase calpain activity mainly as a result of the increased calcium concentration and also a decrease in calpastatin protein abundance.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2020
- Accession Number
- AD1113717
Entities
People
- Amber L Pond
- Gregory H. Hockerman
Organizations
- Purdue University
- Southern Illinois University Carbondale