Early Detection of Lung Cancer Through Molecular Analyses of Single Extracellular Vesicle
Abstract
The overall goal of this project is to advance a new analytical platform tailor-designed to probe single extracellular vesicles (EVs). Specifically, we will develop a new imaging method to probe individual EVs the imaging method will use novel cleavable light-tagged antibodies for repeated probing on same EVs. We will next apply this platform to study EVs at very early stages of lung cancers (LCs) using gold standard genetically engineered mouse models of cancer that naturally arise following genetic mutation in the lung tissue, similar to what may occur in humans. We will monitor both tumor burdens and its molecular characteristics; this information will be compared with single-EV profiling results. The study will work backwards from advanced disease towards increasingly minimal disease, with concordant EV analysis and imaging to understand limits of detection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2020
- Accession Number
- AD1114474
Entities
People
- Hakho Lee
Organizations
- Massachusetts General Hospital