Mechanistic and Therapeutic Implications of Spliceosomal Gene Mutations in ER+ Breast Cancer

Abstract

Recent evidence has revealed that altered mRNA splicing is a mechanism by which tumors can derive constitutive,tumor-promoting signals. Recurrent, somatic mutations in the core RNA splicing factor SF3B1 have been found inseveral malignancies. Through analyses of metastatic breast cancer patients at our center, we have noted hotspotmutations in SF3B1 in up to 6% of cases and these are strongly associated with the ER+/HER2- subtype and inferioroutcomes in patients. We therefore conducted studies to understand the potential implications of SF3B1 mutations onbreast cancer pathogenesis and have so far found that expression of the most common mutant, K700E, leads tocharacteristic alterations in RNA splicing, promotes the invasiveness and lethality of PIK3CA mutant breast cancer, andincreases the sensitivity of PIK3CA mutant breast cancer cells to AKT inhibition. Based on these observations, we arefurther studying the consequences of SF3B1 mutation on breast cancer progression and sensitivity to spliceosomaltargeted therapy.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2020
Accession Number
AD1114508

Entities

People

  • Omar Abdel-Wahab

Organizations

  • Memorial Sloan Kettering Cancer Center

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Blood
  • Breast Cancer
  • Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Clinical Trials
  • Diseases And Disorders
  • Electronic Mail
  • Epithelial Cells
  • Genetics
  • Hematologic Diseases
  • Inhibition
  • Lymphatic Diseases
  • Mammary Glands
  • Medical Personnel
  • Neoplasms
  • New York
  • Proteins
  • Therapy
  • Training

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics