Development of an Experimental Pipeline for Onboarding of Actinobacteria with Rhodococcus ruber C208 as the Example
Abstract
Robust microorganisms that tolerate difficult environmental conditions will be required for bioremediation of Army-relevant materials. The development of synthetic biology tools to leverage organisms with these capabilities has lagged behind the development of tools for traditional microbial chassis organisms. Here, we demonstrate the initial experimental steps for development of novel synthetic-biology chassis organisms using Rhodococcus ruber C208, a member of the Actinobacteria phylum, as an example. We determine the cultivation conditions, antibiotic sensitivity, transformability, and optimal reporter for use in R. ruber C208. Additionally, we sequence the R. ruber C208 genome and examine its ability to grow on diverse organic substrates. These experiments provide the necessary first steps to developing more-sophisticated synthetic biology tools for R. ruber C208 and establish a pipeline for onboarding of novel synthetic-biology chassis organisms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 13, 2020
- Accession Number
- AD1115444
Entities
People
- Caleb Hellman
- Monica Chu
- Nathan Iii D Schwalm
- Randi M Pullen
- Rebecca L. Renberg
- Sean M. Halper
Organizations
- United States Army Research Laboratory