Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines

Abstract

The objective of this project was to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive (T and B cell) responses were measured using molecular and cellular approaches, including RNA sequencing, flow cytometry, and multiplex immunoassays. Overall, this project generated a detailed characterization of immune activation and antigen-specific immune responses in subjects from the clinical trial. Analysis of the data has identified candidate early markers of vaccine immunogenicity which are available to be used in future studies of this vaccine, other dengue vaccines, and vaccines against other viral infections.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2020
Accession Number
AD1116872

Entities

People

  • Alan L. Rothman
  • Jeffrey R. Currier

Organizations

  • University of Rhode Island

Tags

DTIC Thesaurus Topics

  • Adaptive Immunity
  • Antigens
  • B Lymphocytes
  • Blood
  • Cell Physiological Processes
  • Cells
  • Clinical Trials
  • Computational Biology
  • Cytokines
  • Gene Expression
  • Genetic Structures
  • Genetics
  • Immunogenicity
  • Lymphocytes
  • Medical Personnel
  • Systems Biology
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology
  • Oncology and Biomarker-Based Cancer Detection.
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech