Accelerated Innervation of 3D Bioprinted Muscle Construct with Pre-Fabricated Neuromuscular Junctions and Neurotrophic Factor Release System
Abstract
Task 1.1/D1.1 Determine the effects of neurotrophic factors on skeletal muscle development and acetylcholine receptor (AChR) cluster preformation on the bioprinted muscle construct (35% completion during this reporting period, 80% total completion) a. Establishing the optimal conditions of agrin treatment to efficiently induceAChR cluster pre-formation on human muscle progenitor cells (hMPCs) in the bioprinted muscle construct b. Optimizing the bioink composition for 3D bioprinting of muscle constructs with hMPCsc. Determining the viability and differentiation efficiency of hMPCs with agrin treatments in bioprinted constructs d. Establishing the effect of neurotrophic factors (ciliary neurotrophic factor(CNTF) and glial cell line-derived neurotrophic factor (GDNF)) on the hMPC viability and proliferation in the bioprinted muscle construct e. Optimizing the protocol to fabricate PLGA (poly-lactic-co-glycolic-acid) microspheres as a vehicle for controlled release of neurotrophic factors in the bioprinted muscle constructs f. Evaluating the kinetics of neurotrophic factor release from PLGA microspheres in the bioprinted muscle construct.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 25, 2020
- Accession Number
- AD1117128
Entities
People
- James J Yoo
- Ji H. Kim
Organizations
- Wake Forest University