Universal Enrichment Media: A Supportive Technology for Multiplex Detection of Pathogens in Food Matrices
Abstract
Multiplex detection technologies have progressed in increasing sensitivity; however, pathogen enrichment is still required to properly test food for bacterial targets. Here, a universal enrichment media is evaluated along with its ability to successfully enrich S. Typhimurium, E. coli O157:H7, S. flexneri, Y. enterocolitica, E. coli O78:H11, V. cholerae and V. parahaemolyticus for molecular detection in 16 food matrices while in comparison to a standard enrichment media. Results demonstrate that universal enrichment media can perform equally as effective as the standard recommended enrichment media, no significant difference (P greater than 0.05), for the detection of pathogenic bacteria in a variety of food matrices. Next, an alternative methodology is investigated for simultaneous detection based on the combining of separate enrichment aliquots into a single diagnostic run. Results show the successful detection of S. Typhimurium, S. flexneri, V. cholerae, and C. jejuni in water and bok choy using a single diagnostic test after combining aliquots from separate enrichments. This study demonstrates that a universal enrichment media can be as effective as the standard enrichment media for molecular based testing of foods. Further universal enrichment supports the use of multiplex detection for foodborne illness outbreak investigations and food surveillance programs. The mixing of different enrichment aliquots into a single diagnostic run can be used as an effective alternative methodology for simultaneous detection when culture condition requirements cannot be met with a single enrichment media. Advancing enrichment technology to include multiple bacterial targets is complimentary to multiplex detection platforms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 11, 2020
- Accession Number
- AD1117289
Entities
People
- Alma J. Mendoza
- Gianna P. Ahnrud
- Patrick J. Marek