Identifying Biomarkers of Metastasis Through Biosynthetic Tagging
Abstract
Overall work was slowed by closure of labs, suppliers and service providers during the COVID-19 pandemic, and progress on Aim 2 was limited by a technical hurdle related to the mouse model of metastasis. We requested and were granted a no-cost extension to address both issues. We have made progress on the molecular biology proposed in Aim 1, including engineering metastatic and non-metastatic cell lines derived from a murine model of lung adenocarcinoma to stably express the enzymes needed for RNA biosynthetic labeling -- these will be used in wildtype mice. Unmodified cell lines were to be used in transgenic, RNA-labeling mice, but the cell lines were derived from 129Sv mice and dont behave exactly as expected in the transgenic C57BL/6 mice. With separate funding, we are establishing the required transgenic 129Sv mice by crossing the C57BL/6 transgenic mice onto the 129Sv background. We have amended our animal protocol to reflect use of the derived strain and have received local IACUC approval.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2020
- Accession Number
- AD1117671
Entities
People
- Alexander Pertsemlidis
- Yiqiang Zhang
Organizations
- University of Texas Health Science Center at San Antonio