Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein

Abstract

A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered to enhance their properties. In this study, we generated sdAbs specific for Ebola virus envelope glycoprotein (GP) and increased their stability to expand their utility for use in austere locals. Ebola virus is extremely virulent and causes fatal hemorrhagic fever in similar to 50 percent of the cases. The viral GP binds to host cell receptors to facilitate viral entry and thus plays a critical role in pathogenicity.

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Document Details

Document Type
Technical Report
Publication Date
Dec 12, 2017
Accession Number
AD1117776

Entities

People

  • Dan Zabetakis
  • Ellen R Goldman
  • George P. Anderson
  • Jinny L. Liu
  • Lisa C Shriver-Lake

Organizations

  • United States Naval Research Laboratory

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antigens
  • Blood
  • Cells
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Ebola Virus
  • Engineering
  • Fluorescent Dyes
  • Polymerase Chain Reaction
  • Surface Plasmon Resonance
  • Surface Plasmons
  • Viral Hemorrhagic Fevers
  • Virus Diseases
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Nanocomposite Materials Science

Technology Areas

  • Space