Impact of Dopamine Alteration on Brain-Wide Functional Connectivity at Cellular Resolution
Abstract
Widespread neuroplasticity exists in the brain leading to adaptation or maladaptation. Thisproject aims to understand how alteration of dopamine systems, which are profoundly affectedin Parkinsons disease (PD), affects brain-wide plasticity at cellular resolution, employingthe vertebrate model organism zebrafish. During the second year of this project, we haveemployed the transgenic animals that simultaneously express a reporter-tagged enzymenitroreductase (NTR)(for chemogenetic ablation of dopamine neurons to mimic the loss of theseneurons in PD)and GCAMP6s (a genetically encoded calcium indicator for imaging neuronalactivity dynamics). Brain-wide calcium imaging data were collected using head-fixed and tailfreelarval zebrafish preparation. The loss of tyrosine hydroxylase+ (TH+) neurons wasquantified in different locations throughout the brain that revealed a range from 20% to 100%neuronal loss in different brain locations. Computational tools for brain registration,neural activity and tail movement analysis were further refined. Together, these resultspoise us to uncover how the loss of TH+ neurons in larval zebrafish affect brain-wide neuralactivity dynamics.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2020
- Accession Number
- AD1120273
Entities
People
- Su Guo
Organizations
- University of California Regents