Transcription Factor Analysis of SLE
Abstract
The goals of this project are to understand the regulation of genes with differential expression in SLE. We are using ChIP-seq to define the epigenetic landscape that sets the transcriptional capacity for expression and we are using RNA-seq to define dysregulated expression. Bioinformatic approaches will extract transcription factor binding sites from regions of altered histone modifications. We will then alter those transcription factors to define effects on cell behavior. Due to COVID-19, we have begun recruitment and ChIP-seq/RNA-seq sample preparation but have not yet run any libraries. With accelerated recruitment, we will still be able to complete our proposed studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2020
- Accession Number
- AD1120986
Entities
People
- Kathleen E. Sullivan
Organizations
- Children's Hospital of Philadelphia