Next-Generation Human Prostate Cancer Cocultures to Optimize Autologous Chimeric Antigen Receptor T-Cell Immunotherapy
Abstract
In this reporting period, we have optimized a methodology to process fresh primary humanprostate cancer tissues obtained at standard-of-care prostatectomy and establish viabletissue slices that can be cultured for up to a week. Our preliminary studies indicate thatthe prostate cancer tissue slices can be maintained without a loss of cell viability or grossdisruption of tissue architecture during this time period. Immunohistochemical studies showedmaintenance of androgen receptor, prostate stem cell antigen, and Ki67 expression in thesetissue slices. We have developed a multiparametric flow cytometry panel to examine changes inthe tumor microenvironment of the tissue slices over time. We are now optimizing enzymaticdigestion protocols to dissociate tissue slices to single cells to maximize cell recovery forthese analyses. Once this technical optimization and characterization is complete, we intendto move forward with immunological co-culture studies using chimeric antigen receptor T cellsand human primary prostate cancer tissue slices.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2020
- Accession Number
- AD1121018
Entities
People
- John K Lee
Organizations
- Fred Hutchinson Cancer Center