Early Intervention Stem Cell Based Therapy (EISCBT) for Corneal Burns and Trauma
Abstract
The goal of this project is to validate new stem cell markers which can be used to assess the corneal stromal stem cell (CSSC) regenerative potential and develop a regenerative corneal bandage (ReCoBand) with CSSCembedded in or with a regenerative extracellular matrix that can be applied in the battlefield to prevent permanent scarring of the cornea and treat the corneal scarring after trauma, blast, or burn wounds to preserve vision. In the first year of this project we have: (1) validated stem cell markers with which CSSC possess the regenerative potential, (2) characterized a transgenic mouse model which the fibrotic cells turning to green when the cornea isscarred, (3) confirmed that CSSC can prevent corneal scarring on this transgenic Col3a1-EGFP mosue model, (4) discovered one of the mechanisms that CSSC prevent corneal scar formation, (5) proved that ReCoBand withverified CSSC can prevent corneal scar formation in an alkali burn rabbit model. These accomplishments meet the proposed milestones and predict success in meeting the overall goals of the project by the end of the 2 year timecourse.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2020
- Accession Number
- AD1121797
Entities
People
- Yiqin Du
Organizations
- University of Pittsburgh