A Two-Step Phage Display Panning Method for Selecting Peptides to Detect HMGB1 and its Potential for Developing Peptide-Based Biosensors
Abstract
Expeditionary and deployed personnel are exposed to the widest variety of biological, chemical, environmental, and biomedical threats. Biosensors, devices that combine a biological element with a technological sensor platform to detect an analyte, have great promise and are urgently needed to detect and identify threats in real-time. Unfortunately, biosensors are hampered by a historical reliance upon animal-generated antibodies as their biological recognition elements. Antibody production and characterization are slow, difficult, and sequential processes that rely upon inducing an immune response in a laboratory animal. We characterized an alternative method for producing biological recognition elements: phage display. Phage display leverages small viruses (bacteriophages) which display short, random peptides. We intended to develop a phage panning protocol that excludes phages with peptides that bind a background solution and collects only those which bind a desired analyte. This two-step panning scheme can be tuned to include almost any analyte or background solution. It produces many unique peptides with desirable characteristics for use as biological detection elements in biosensors. The entire pipeline can be completed in approximately ten weeks and the peptides it yields have many advantages as recognition elements in biosensors when compared to more traditional, antibody-based elements.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 28, 2020
- Accession Number
- AD1123792
Entities
People
- Amanda M. Bache
- April A. Ford
- David J. Lemon
- Eun Y. Huh
- Holly C. May
- Steven X. Moffett
- Yoon Y. Hwang
Organizations
- Naval Medical Research Unit—San Antonio