Molecular CrossTalk: Bone Metastatic Prostate Cancer and Nociceptive Neurons
Abstract
The goal of this project is to determine the roles of angiotensin II and its receptor in prostate cancer induced bone pain and bone metastatic growth. Aim 1 will provide the framework to identify the extent to which the interaction between cancer cells and nociceptive neurons through the angiotensin II and receptor axis affects cancer-induced bone pain. Aim 2 will determine the downstream molecular mechanisms whereby angiotensin II and its receptor axis affect bone pain. Aim 3 will define how nociceptive neuron influence tumor outgrowth. We believe that the insights derived from our investigations will lead to new strategies for reducing cancer-induced bone pain and also the outgrowth of bone metastasis. During this period, we further characterized a syngeneic murine prostate cancer (PCa)-induced bone pain model which allows us to further elucidate the roles of the crosstalk between bone metastatic PCa and sensory neurons in both bone metastatic progression and its resultant bone pain in an immunocompetent setting. We tested and refined methods for deep tissue clearing and 3D light sheet imaging of intact mouse bone for future investigation of cellular interactions between cancer cells, immune cells and sensory neurons. We also began to identify the roles of cancer-derived angiotensin Il in the nerve growth in the bone metastatic setting.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2020
- Accession Number
- AD1125470
Entities
People
- Christopher Peters
- Yusuke Shiozawa
Organizations
- Wake Forest University