Pilot Study of Extracorporeal Low-Flow Filtration in a Porcine (Sus scrofa) Model of Sepsis
Abstract
One new therapy for treating sepsis in critical illness is the Seraph-100 (ExThera Medical, Martinez, CA). However, a limitation of the device is that it requires a large bore catheter and high blood flows. We hypothesized that adapting the technology for a low-flow state could allow for smaller catheters to be utilized. Our main objective was to assess the feasibility of low-flow extracorporeal blood purification in a swine (Sus scrofa) model of sepsis. Methods: Sepsis was induced by infusing live Pseudomonas aeruginosa (ATCC cell line 27853) into 12 Yorkshire swine weighing 60-80 kgs. Animals either underwent low-flow blood purification with either that Serpah-100 or control. Blood cultures were done and quantified at the start of blood purification, 3 hours later, and 6 hours later. Arterial blood gases and lactate were done every 30 minutes during the procedure. Results: The model was developed and the approach was determined to be feasible. We found a catheter that could be used for the purposes of low-flow extracorporeal blood purification and perfected techniques for sepsis induction and quantification of bacteremia in swine. Conclusion: Studying low-flow extra corporeal blood purification is feasible in this models of sepsis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 04, 2021
- Accession Number
- AD1126205
Entities
People
- Ian J Stewart
Organizations
- David Grant USAF Medical Center