New Humanized Mouse Model (DRAGA: HLA-A2. HLA DR4.Rag1KO.lL2R gammaCKO.NOD) to Generate and Test Therapeutic Human Anti-Flu Monoclonal Antibodies
Abstract
Over the past century, four different influenza pandemics have occurred resulting innumerous fatalities around the globe. Given the uncertainties of a new influenza virus, new preventive measurements and treatments need to be developed. Antibody-based therapy has been effectively used before to treat infectious diseases, including respiratory syncytial virus infection. Neutralizing antibodies play an important role in protection from influenza virus infection by recognizing its major surface glycoprotein: hemagglutinin (HA). The recently developed DRAGA (HLA-DR4.RagKO.IL2RcKO.A2.NOD) mouse model expresses the human MHC class I molecule HLA-A2 in combination with the human MHC class II molecule HLA-DR4.DRAGA mice can produce all immunoglobulin subtypes and elicit high titers of specific human antibodies against an infectious agent. The aim of this work is to generate and test the efficacy of neutralizing hu-mAbs targeting a conserved HA region using DRAGA mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 06, 2017
- Accession Number
- AD1128325
Entities
People
- Mirian I. Mendoza
Organizations
- Uniformed Services University of the Health Sciences