Mechanisms of Resistance to Immunotherapy in Osteosarcoma

Abstract

Osteosarcoma is a cancer that primarily affects children, adolescents, and young adults. Sofar, treatment of this cancer with immunotherapy has been unrewarding. However, themechanisms that mediate resistance to immunotherapy are poorly understood. Our goal is todetermine whether osteosarcoma-derived exosomal microRNAs inactivate immune cells in or nearthe tumor environment. We have found that CD28 might be a target through which osteosarcomasrestrain T cell activation, although different mechanisms might mediate this effect in tumorsfrom humans and from dogs. Overall, the transcriptional programs in osteosarcoma, includingthose of immune cells in the microenvironment, are controlled by epigenetic mechanisms thatinclude chromatin modification (methylation) and microRNA-dependent RNA degradation. A majorinnovation from our work is the development of a novel molecule that simultaneously inhibitsmyeloid and exhaustion immune checkpoints, and which may have the capacity to mitigateosteosarcoma-mediated immunosuppression. This approach could change the paradigm of howimmunotherapy is used for this and other solid tumors.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2020
Accession Number
AD1128351

Entities

People

  • Jaime F. Modiano

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Colon Cancer
  • Culture Techniques
  • Cultured Cells
  • Data Analysis
  • Disease Attributes
  • Genetics
  • Health Services
  • Immunomodulation
  • Lymphatic System
  • Lymphocytes
  • Medical Personnel
  • Oncology
  • Sarcoma
  • Stem Cells
  • Therapy
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Canine Service Warrior Training Program for Wounded Warriors in the Veterinary Industry, Supported by Donors.
  • Molecular Biology and Genetics
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech