Prognostic Selection of Osteogenic Stem Cells Using Label-Free Microfluidic Separation

Abstract

Our central hypothesis is that while mixed adipose derived stem cells (ADSCs) fail to induce bone formation, the smallest sized ADSCs with the least granularity, that can be isolated in a pure form using microfluidic separation, will induce bone formation reliably and consistently. We seek to develop a label-free, high-throughput, microfluidic isolation platform for the isolation of adipose derived stem cells (ADSCs), without the need for surface markers. In the period under report, we have compared biophysical characteristics of a bone forming (positive control) stem cells population with mixed ADSCs and based on that we have successfully devised microfluidic platform to isolate the sub-population of ADSCs that will possess reliable bone-forming potential.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2021
Accession Number
AD1129286

Entities

People

  • Abhijit S. Dighe
  • Nathan S. Swami
  • Quanjun Cui

Organizations

  • University of Virginia

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical Research
  • Bone Marrow
  • Bone Regeneration
  • Bones
  • Cells
  • Computational Fluid Dynamics
  • Diameters
  • Displacement
  • Fluid Dynamics
  • Frequency
  • Growth Factors
  • Histograms
  • Impedance
  • Maryland
  • Osteogenesis
  • Peptide Growth Factors
  • Platforms
  • Stem Cells
  • Tissues
  • United States
  • Virginia

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biology
  • Nanoscale Plasmonic Nanotechnology
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology