Immunohistochemical Phenotype Analysis of Cells Associated with Pulpal Mineralized Extracellular Matrix in a Porcine Endodontic Regeneration Model
Abstract
Introduction: Early apical mineralization responses following regeneration therapy remain to be fully characterized, partially due to limited availability of larger animal models. The aims were to evaluate a young porcine model for the study of endodontic regenerative treatment, and to compare via immunohistochemistry the early cellular and mineralizing events occurring at the apex following therapies utilizing BiodentineTM (Septodont, Saint Maur des Fosses, France) and Pro-Root MTATM(Dentsply Sirona, Tulsa, OK).Methods: Immunohistochemical techniques were used to characterize cell populations and mineralized tissues in samples previously obtained from a study utilizing a young porcine model for endodontic regeneration in which mandibular first and second premolars in five pigs (3 months old) received regenerative treatment. Teeth were divided into five treatment groups: One: treated with blood clot without bioceramic; Two: treated with blood clot and MTA; Three: treated with blood clot and Biodentine; Four: treated with MTA without blood clot (positive control); and Five: no treatment (positive/negative control). Samples were harvested after a three-week healing period, fixed in formalin, evaluated radiographically, and processed for histology. Sections were stained immunohistochemically for mineralized matrix markers,2including dentin sialophosphoprotein, osteocalcin, osteopontin, cementum-attachment protein, osterix, and proliferating cell nuclear antigen. Results: The blood clot treatment groups and MTA/Biodentine treatment groups demonstrated extensive areas of active hard tissue matrix formation on the canal walls, resembling cellular cementum-like deposition extending from the apex, with high levels of cementoblast-like cell activity. Conclusions: A cementum-like mineralization of potential clinical benefit can occur in the apical root region following endodontic regenerative treatment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 13, 2018
- Accession Number
- AD1132009
Entities
People
- Joshua D. Pruden
Organizations
- Uniformed Services University of the Health Sciences