Nanotopographical Influence on Cell Behavior
Abstract
The wound healing process is a complex cascade of events, recruiting different cell types that receive precise instructions through a variety of physical cues. One such cue involves cellular response to the underlying substrate topography, ranging from the nano- to microscale. However, the fundamental mechanisms throughout this process are still poorly understood, largely due to the fact that cellular response in-vitro is extremely context dependent. Additionally, heterogeneity amongst experimental platforms further confounds results between research groups. Here, we outline a novel fabrication method to impart highly reproducible nanotopographical cues in SiO2 coverslips that are readily integrated with all forms of live-cell microscopy, can easily scale up for manufacturing, and are well characterized in terms of surface roughness and protein adsorption. The SiO2 topography chips are validated on fibroblasts, a crucial cell type in the wound healing process, and fibroblast adhesion and migration in response to topographical cues is investigated.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 25, 2021
- Accession Number
- AD1134184
Entities
People
- Michael C. Robitaille
Organizations
- United States Naval Research Laboratory