Validation of Advanced Molecular Biological Tools to Monitor Chlorinated Solvent Bioremediation and Estimate cVOC Degradation Rates

Abstract

This demonstration had three specific objectives. The first objective was to demonstrate the utility of quantitative proteomics (qProt) to measure the absolute abundance of Dhc reductive dechlorination biomarker proteins in laboratory-controlled microcosms with various Dhc cell titers. Contaminant concentration and ethene measurements over time were used to determine cis-DCE and vinyl chloride (VC) reductive dechlorination rates. The second objective was to correlate observed degradation rates with Dhc biomarker gene and protein abundances. The successful completion of objectives 1 and 2 led to a go/no-go decision point before conducting demonstration/validation efforts of the qProt approach at military sites impacted with chlorinated ethenes.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 24, 2020
Accession Number
AD1135013

Entities

People

  • Craig Bartling
  • Fadime K. Murdoch
  • Frank Loeffler
  • Jayda Meisel
  • John A. Wilson
  • Jonathan Istok
  • Kate Kucharzyk
  • Mandy Michalsen
  • Paul B. Hatzinger

Organizations

  • United States Army Corps of Engineers

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkenes
  • Biodegradation
  • Chemical Analysis
  • Chemical Synthesis
  • Chemistry
  • Ecology
  • Environment
  • Environmental Monitoring
  • Fatty Acids
  • Gas Chromatography
  • Liquid Chromatography
  • Mass Spectrometry
  • Measurement
  • Microbiology
  • Microorganisms
  • Organic Chemistry
  • Regression Analysis

Fields of Study

  • Environmental science

Readers

  • Groundwater Contamination Remediation.
  • Gulf War Illness and Chronic Multisymptom Illness in Veterans.

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation