Gut and Cartilage Microbiomes as Novel Mediators of Inflammation in and Potential Therapy for Post-Traumatic Osteoarthritis
Abstract
In this study we plan to evaluate both the pathogenic and potential protective effects of changes in body microbiomes along with their interactions with the immune system in PTOA, using cutting-edge microbiome and immune cell subtyping analyses. In Aim 1 (year 1), we will evaluate correlations between gut and cartilage microbiomes in PTOA-susceptible C57BL/6J (B6) and PTOA-resistant MRL/MpJ mice (MRL) before and after DMM-induced PTOA, and correlate these findings with systemic inflammatory cell responses via peripheral blood cell phenotyping. In Aim 2 (year 2), we will establish the direct contributions of the gut microbiome in both establishment of a cartilage microbiome and in PTOA pathogenesis by introducing the gut microbiota of B6 and MRL mice into germ-free (GF) mice, followed by DMM. In Aim 3 (year 2), we will examine the potential therapeutic potential of altering the gut microbiota as a preventative / therapeutic for PTOA by conducting gut microbiome transplantation of superhealer MRL into non-healer B6 mice, measuring susceptibility to OA, and quantifying associated systemic inflammatory cell changes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2021
- Accession Number
- AD1135306
Entities
People
- Matlock A. Jeffries
Organizations
- Oklahoma Medical Research Foundation