Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay

Abstract

Pollinators provide essential ecosystem services and are critical components of resilient ecosystems. Yet, pollinators are suffering staggering global declines leading to increased listing under the Endangered Species Act. This presents challenges for DoD installations. The technical objectives of this project were to: 1) develop and validate broad-spectrum pollinator eDNA metabarcoding primers, 2) test the efficacy of multiple capture methods to obtain Next Generation Sequencing-ready pollinator eDNA from flowers, and 3) validate eDNA sampling and microfluidic metabarcoding methods in controlled and field experiments with mixed plant/pollinator communities.

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Document Details

Document Type
Technical Report
Publication Date
Apr 06, 2020
Accession Number
AD1135346

Entities

People

  • Brenda Molano-flores
  • Joseph Benito
  • Lynsey R Harper
  • Mark M. Davis
  • Matthew L. Niemiller

Organizations

  • Illinois Natural History Survey

Tags

DTIC Thesaurus Topics

  • Birds
  • Cells
  • Chemical Reactions
  • Computational Biology
  • Data Analysis
  • Deoxyribonucleic Acids
  • Department Of Defense
  • Ecology
  • Entomology
  • Eutrophication
  • Fish
  • Gel Electrophoresis
  • Habitats
  • Lepidoptera
  • Pests
  • Ribonucleic Acids
  • Spiders
  • United States
  • Wildlife

Fields of Study

  • Environmental science

Readers

  • Distributed Systems and Data Platform Development
  • Molecular Genetics
  • Parasitology and Pharmacology of Malaria.