SELECT: Selecting Elite-performers with Longitudinal Epigenomic Characterization and Tracking

Abstract

The major goal of this work is to generate longitudinal epigenomic datasets for a group of individuals going through an elite selection process. Blood will be drawn from individuals going through elite selection at two times points, peripheral blood mononuclear cells (PBMCs) will be isolated and frozen, and these samples will be sent to JCVI for DNA methylation sequencing. The PMCs will be counted and accessed for quality and viability. DNA will be extracted from PBMCs that pass quality control for DNA methylation sequencing. DNA will be bisulfite converted to detect methylated bases and sequenced. Serum samples will be used to measure the metabolome. DNA methylation calls and metabolic results will be delivered.

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Document Details

Document Type
Technical Report
Publication Date
Nov 30, 2020
Accession Number
AD1137311

Entities

People

  • Todd P Michael

Organizations

  • J. Craig Venter Institute

Tags

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  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Abstracts
  • Agreements
  • Air Force
  • Biomedical Information Systems
  • Cell Count
  • Classification
  • Computational Biology
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  • Databases
  • Extraction
  • Genetic Structures
  • Information Operations
  • Inventions
  • Law
  • Methylation
  • Military Research
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  • Patents
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Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Immunology
  • Molecular and genetic basis of cancer.