Novel Aptamer-Based Biosensor Platforms for Detection of Cardiomyopathy Conditions
Abstract
There is a need for a point-of-care biosensing device for rapid screening and monitoring of cardiomyopathy conditions and progression to lower incidence, death occurrences, and healthcare costs. Overall aim of this project is to create a cardiomyopathy condition screening and monitoring tool to simplify the current biochemical marker testing procedures by developing vertically aligned platinum wire aptamer-based multi-array biosensor for precise, accurate, reliable and rapid measurement of the presence of relevant cardiac marker levels in the human whole blood and serum using electrochemical impedance spectroscopy. This work thus far, has demonstrated how to construct an impedimetric multi-array biosensor platform based on platinum wires functionalized with aptamers, and has successfully navigated the platform all the way beginning from construction to optimization and validation of feasibility in biological samples. Initially, we optimized the optimal platinum wire diameter and surface finish which was necessary to create a biosensor that does not experience saturation within the acceptable clinical ranges of brain natriuretic peptide (BNP) and troponin T (TnT) antigens, the accepted cardiac biomarkers. Following validation, the focus then shifted into assessing the self-assembled monolayer (SAM) approach utilized to tether the BNP and TnT specific aptamers to the electrode surface, determining both the optimal incubation time and concentrations necessary for each layer as well as assessing the necessity of each layer. The best self-assembled-monolayer (SAM) combination that provided reliable, accurate and most sensitive response was determined to be Platinum Cysteamine-Glutaraldehyde-Neutravidin-Aptamer and this SAM combination showed excellent precision, reasonable sensitivity, and stable insulation of the linker proteins that can easily interfere with the biosensor readings.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2021
- Accession Number
- AD1139916
Entities
People
- Abhijit Roy
- Moni K. Datta
- Prashant N Kumta
- Sangeetha Kunjukunju
Organizations
- University of Pittsburgh