Evaluation of the Biomeme Franklin Human Performance Factors

Abstract

U.S. Army Combat Capabilities Development Command Chemical Biological Center (CCDC CBC) scientists completed an evaluation of the human performance factors related to the execution of COVID-19 testing using the SARS-CoV-2 Test Kit including (1) ease of use, (2) workflow development and assessment, and (3) time to results and sample throughput. The assay was found to require a fairly complex laboratory in order to safely process the samples due to the need for a biosafety cabinet to contain any SARS-CoV-2 containing aerosols. The workflow required nearly 3.5 h to complete 7 extractions, set up the seven RT-PCR reactions plus the two control RT-PCR reactions, run the RT-PCR on the Biomeme Franklin thermocycler, and interpret results. Therefore, throughput was estimated at 42 samples per 24 period on a single Franklin thermocycler.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2020
Accession Number
AD1143390

Entities

People

  • Bryan A. Rivers
  • Kimberly L. Berk
  • Patricia E. Buckley
  • Steven M. Blum

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biosafety
  • Calibration
  • Covid-19
  • Distance Learning
  • Instruction Manuals
  • Instructions
  • Job Training
  • Judgment
  • Manuals
  • Materials
  • Mobile Application Software
  • Mobile Phones
  • Motor Skills
  • Personal Protective Equipment
  • Polymerase Chain Reaction
  • Protective Equipment
  • Quality Control
  • Sars
  • Scientists
  • Smartphones
  • Thermal Cyclers
  • Training
  • Virus Diseases

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  • Nuclear and Radiation Engineering.