Silent Guardian at NRL October 2004-March 2005
Abstract
In Nov 2004, NRL and the USAF Surgeon General accelerated the development of an emergent resequencing microarray system per direction of the Deputy Assistant to the Secretary of Defense, Nuclear, Chemical and Biological Programs. This early demonstration project was called Silent Guardian (SGD). During SGD approximately 5,000 RPM assays were performed using patient samples from six MTFs and spiked normal nasal wash, demonstrating: (1) a capability to establish a full production, high throughput CLIA quality analysis laboratory using high density resequencing microarrays in under 2 months, (2) a capability to integrate disparate data systems for collection of clinical data, tracking analysis and reporting the result to geographically dispersed PIs, (3) that hundreds of diverse genetic sequences for common respiratory pathogens causing illness in the general population could be obtained and unequivocally identified in approximately 18-19 hours, and (4) that the system was capable of sensitive detection and discrimination of a panel of biothreat agents and discriminated from nearly identical non-pathogenic organisms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 29, 2021
- Accession Number
- AD1143810
Entities
People
- Anne W. Kusterbeck
- Anthony P. Malanoski
- Chris R. Taitt
- David A. Stenger
- Frances Ligler
- Joel M. Schnur
- Lisa C Shriver-Lake
- Steve K. Pollack
Organizations
- United States Naval Research Laboratory