Inflammation and the Malignant Transformation of Endometriosis
Abstract
We are conducting a study to examine the association between inflammatory markers in peritoneal fluid and driver mutations and immunohistochemical (IHC) markers of cell proliferation and invasiveness in endometriosis tissue. We will also evaluate whether inflammation-related epidemiologic factors and systemic inflammation (e.g., CRP, IL-6 plasma levels) are associated with inflammatory markers in peritoneal fluid. Data and specimens for this study have been previously collected from A2A cohort, a longitudinal cohort of women over sampled for those surgically diagnosed with endometriosis. This project consists of selecting appropriate individuals with peritoneal fluid, endometriosis tissue, blood, and epidemiologic data, accessing samples for bio specimen assays, and conducting analyses. Targeted sequencing is being used to identify our genes of interest, ELISA is being used to measure inflammatory biomarkers in peritoneal fluid and blood, and RNAseq to assess the tissue expression of selected markers. To date we have conducted the assays to measure IL-6, IL1b, TNFR2 in blood. Peritoneal fluid samples are at the laboratory and are currently being assay. We have also successfully completed our pilot study of endometriosis tissue DNA/RNA extractions and sequencing and are currently pulling tissues from the biorepository to be send for DNA/RNA extraction. Our work this passed year was delay to lab shut downs due to the COVID-19 pandemic but now that labs are running again we are making progress towards having all of the necessary lab work done and when it is complete we will start data analysis.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2021
- Accession Number
- AD1144311
Entities
People
- Holly R Harris
Organizations
- Brigham and Women's Hospital
- Fred Hutchinson Cancer Center
- Michigan State University