Modulating Cancer Genetics for Immune Regulation and Breast Cancer Therapy

Abstract

In contrast to its important function in DNA repair, overexpression of human MSH2/6 mismatch recognition protein is driving the progression of basal like breast cancer and is associated with poor prognosis. This project will establish the molecular mechanisms of this MSH2/6 activity. During the first funding period we have established an experimental workflow to test the hypothesis that MSH2/6 protein targets methyltransferase DNMT1 to specific promoters and activates its enzymatic activity thus connecting the MSH2/6 overexpression with altered epigenetic regulation. Our preliminary data suggest that this hypothesis is correct. During the next funding period we will complete the biochemical and single-molecule studies testing this hypothesis.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2021
Accession Number
AD1144312

Entities

People

  • Maria Spies

Organizations

  • University of Iowa

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biochemistry
  • Biology
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Covid-19
  • Developmental Biology
  • Genetics
  • Molecular Biology
  • Molecules
  • Sequences
  • Small Molecules
  • Therapy
  • Total Internal Reflection

Fields of Study

  • Biology

Readers

  • Agricultural Chemistry/Soil Science
  • Molecular Biology and Genetics
  • Molecular Genetics

Technology Areas

  • Biotechnology