Genomic Surveillance of SARS-CoV-2
Abstract
SARS-CoV-2, the virus that causes COVID-19, emerged in December2019 and created a global pandemic resulting in nearly 3.5 million deaths worldwide. In the Department of Defense (DoD), over 290,000 personnel (civilians, dependents, and active duty) have been infected with the virus to date. To enhance military readiness, the CIRS Laboratory developed a sequencing and bioinformatics workflow for molecular epidemiological SARS-CoV-2 surveillance using excess clinical specimens collected under IRB protocol FWH20200103E at Lackland AFB. This workflow includes viral RNA isolation, viral load quantification, tiling-based next generation sequencing, bioinformatics analysis, and data visualization via phylogenetic trees and protein mapping.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2021
- Accession Number
- AD1145114
Entities
People
- Ashley Nazario-Toole
- Holly M. Nguyen
- Hui Xia
- Thomas F. Gibbons
Organizations
- 59th Medical Wing