Evaluating Senescent Stromal Fibroblasts as a Promoter of Prostate Cancer Lethality to Inform a Paradigm Shift in Prognostic, Predictive, and Therapeutic Strategies
Abstract
We are conducting multidisciplinary research to address the hypothesis that senescent prostate stromal fibroblasts, especially those with the senescence-associated secretory phenotype (SASP), promote lethal prostate cancer, and are more prevalent in Black men. Despite the pandemic, our research team continued non-in person lab-based aspects of the SOW, including study designing, and identifying new strategies to overcome limitations and to optimize the measurements. We received IRB approval. We developed the Prostate Cancer Case-Cohort Study using PCPT linked with Medicare claims. The study includes 151 cases and a subcohort of 378 men at risk (2.5x case N). The subcohort has a median age of 69 years and median PSA at the end-of-study biopsy of 1.5 ng/mL. We selected variables for the analysis, and requested biopsy tissue from the PCPT biorepository. SWOG and JHH are negotiating the MDUA. We requested and received test prostate tissue to use to optimize the multiplex staining and imaging protocols; had the specialized equipment we use serviced and replaced the illumination system to ensure measurement quality; identified strategies to reduce autofluorescence for our IF/imaging work; identified a possible new commercial technology. The Aim 3 question (progression to mets after salvage tx for recurrent disease) will benefit from a newly identified secondary analysis of a subset of the Aim 2 study (progression to lethal disease after prostatectomy) made possible by subsequent re-linkage extending follow up of men who experience biochemical recurrence from 2004 to 2018. Barriers to completing the Year 1 SOW included institutional restrictions on non-COVID lab research and on hiring/on-boarding during the pandemic. Year 1 was fully during the pandemic, nevertheless, we made good progress on key aspects of the SOW
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2021
- Accession Number
- AD1145736
Entities
People
- Elizabeth A Platz
Organizations
- Johns Hopkins University