Lateral Flow Immunoassay Development for Multiplex Detection of Waterborne Pathogens Utilizing Surface Enhanced Raman Scattering (SERS) Reporter-Encapsulated Nanoparticles

Abstract

Rapid detection of waterborne bacterial pathogens implicated in infectious diseases such as cholera, dysentery, shigellosis and typhoid fever is of great importance to public health. Timing is the limiting factor for pathogen detection as traditional diagnostic assays require a minimum of 24 hours as well as extensive resources and expertise. Our objective is to develop a paper-based lateral flow immunoassay (LFA) platform for the detection of multiple waterborne pathogens. Multiple labeling strategies will be compared, including SERS-based and fluorescence-based labels.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2021
Accession Number
AD1146886

Entities

People

  • Amber Mallory
  • April Cadena
  • Joseph L. Spano
  • Luis A. Martinez

Organizations

  • 59th Medical Wing

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Antibodies
  • Bacteria
  • Calibration
  • Cardiovascular Physiological Phenomena
  • Department Of Defense
  • Detection
  • Escherichia Coli
  • Governments
  • Gram-Negative Bacterial Infections
  • Heart Rate
  • Infectious Diseases
  • Nanoparticles
  • Pathogenic Bacteria
  • Photographs
  • Public Health
  • Raman Scattering
  • Raman Spectra
  • Raman Spectroscopy

Readers

  • Microbial Pathology
  • Nanoscale Plasmonic Nanotechnology
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology