Cell-Free DNA for Diagnosis of Malignancy, Genetic Mutations and Prognosis in Lung Cancer Related Malignant Pleural Effusions
Abstract
The objectives of this project is to assess if cell-free DNA in pleural effusions of patients with lung cancer can help establish diagnosis, assess targetable genetic mutations, and response to therapy. For aim 1, we recruited 20 patients with benign pleural effusions and30 patients with lung cancer pleural effusions. Based on cfDNA, we determined integrity index as the ratio of concentration of long (247 bp) to short (115 bp) DNA fragments. The mean integrity index of benign vs. malignant pleural effusions was 0.29 vs. 0.42, t-test P value of 0.01.For aim 2, we determined targetable mutations using cfDNA in 30 patients with lung adenocarcinoma pleural effusions. With next generation sequencing, we were able to detect mutations in 26 out of 30 patients. For aim 3, we have recruited 13 patients with lung cancer pleural effusions. Once, we have recruited the target 30 patients, we will determine if cfDNA can inform about the response to therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2021
- Accession Number
- AD1150355
Entities
People
- Kamran Mahmood
Organizations
- Duke University