20 L Scale Once-Fed-Batch Process Characterization of Magnetospirillum Gryphiswaldense Strain MSR-1 for Production of Magnetosomes
Abstract
Magnetite nanoparticles (mNPs; chains of magnetic crystals found in prokaryotes), especially the biomembrane-enclosed form of bacterial nanoparticles known as magnetosomes, have received keen commercial interest because of their narrow size range and good dispersibility. The biomineralization of magnetosomes is a highly controlled process regulated at the gene level that results in high-purity, single-magnetic-domain particles (i.e., magnetite [Fe3O4] or greigite [Fe3S4]). Because of the highly restrictive culture condition for magnetotactic bacteria in terms of slow growth, dissolved oxygen, and nutrient requirements, the yields of both magnetosomes and their host microorganisms under laboratory cultures tend to be low. In this effort, Magnetospirillum gryphiswaldense strain MSR-1 was used in a 20 L fermentor system to initially characterize the dissolved oxygen conditions for induction of magnetosome formation, establish a baseline magnetosome yield, and explore ways to increase the magnetosome yield over baseline culture conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2021
- Accession Number
- AD1152056
Entities
People
- Alena Calm
- Frank J. Kragl
- Kelley Betts
- Michael H. Kim
Organizations
- United States Army Combat Capabilities Development Command