Gene Therapy for Catecholaminergic Polymorphic Ventricular Tachycardia

Abstract

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a inherited arrhythmia syndrome characterized by life-threatening arrhythmias during times of stress or exercise. Dominant mutations in the intracellular calcium (Ca[exp 2+]) release channel RYR2 are responsible for the majority of clinical cases. Despite maximal medical therapy, patients continue to have breakthrough events or therapy related complications. To response to this unmet clinical need, we have developed a targeted gene therapy to suppress arrhythmias by inhibiting the Ca[exp 2+] regulated kinase CaMKII. Using adeno-associated virus (AAV) vectors we demonstrated efficacy in cellular and animal models of CPVT by targeted expression of CaMKII peptide inhibitors. This grant proposal is focused on the further refinement and testing of a clinical CaMKII peptide inhibitory vector in preparation for a human clinical trial. During this granting period we have determined the cardiac-specific promoter for optimal transgene expression and laid the foundation for refinement of the peptide inhibitor. We have also expanded our clinical network of CPVT patients and performed analysis of current healthcare costs for CPVT management.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2021
Accession Number
AD1152438

Entities

People

  • Vassilios Bezzerides
  • William T. Pu

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Arrhythmia
  • Biomedical Research
  • British Columbia
  • Cardiac Arrhythmias
  • Carrier Proteins
  • Clinical Trials
  • Corporations
  • Diseases And Disorders
  • Gene Therapy
  • Genetics
  • Health Services
  • Medical Personnel
  • Monitoring
  • Patent Applications
  • Production
  • Proteins
  • Standards
  • Therapy
  • Viral Structures
  • Viruses

Fields of Study

  • Biology
  • Medicine

Readers

  • Cardiovascular Physiology
  • Molecular Genetics
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech