Transfer RNA-Derived Fragments as Novel Diagnostic and Functional Targets for Malignant Mesothelioma
Abstract
This project explores the potential for small fragments of genetic material called transfer RNA-derived fragments (tRF)s as novel diagnostic, early disease and disease progression biomarkers as well as targets for therapy in malignant mesothelioma. In the first 12 months of the study most of the research effort was in optimizing the methodology for sample preparation, given that new methods have been commercially developed, and preparation and measurement of tRF levels in two patient cohorts. The data for the first patient cohort of 400 samples are currently being analyzed by a biostatistician. The second cohort of 500 samples from the US is being prepared for analysis. This study will also examine functional roles these tRFs play in mesothelioma cells and their effect on tumor growth which may help identify new therapeutic approaches for this disease. In the first 12 months we have concentrated on examining tRF levels in mouse and human mesothelioma cells to determine which cell lines we will utilize in the functional aims of the study in the second 12 months.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2021
- Accession Number
- AD1152669
Entities
People
- Cecilia Prele
- Nick De Klerk
- Steven Mutsaers
- William Musk
Organizations
- University of Western Australia