In Vivo and Ex Vivo Models to Study Ischemia/Reperfusion Injury, Endothelial Cell Protection, and Limb Preservation in a Prolonged Field Care Scenario
Abstract
In this project, endothelial cell protection and extracorporeal machine perfusion will be combined to achieve a massive prolongation of the time between traumatic amputation and replantation. In the first year, Milestone 1: 'Establishment of ex vivo - in vivo models for extremity I/R injury in a PFC scenario' was reached and work on Milestone 2: 'Validation of ex vivo - in vivo models for the effect of EC protection on extremity I/R injury in a PFC scenario' was started. In particular, a massively increased I/R injury was observed in pig limbs perfused after 9 h ischemia as compared to less than 1 h, both in extracorporeal and in vivo limb perfusion. Currently available analyses include limb weight gain, wet/dry ratio of muscle tissue, perfusion blood electrolytes and lactate, as well as immunofluorescence analysis of dystrophin patterns for muscle tissue integrity. Despite a 4-month lockdown of animal experimentation due to the COVID-19 pandemic the project did not incur a major delay until today.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2021
- Accession Number
- AD1152707
Entities
People
- Robert Rieben
Organizations
- University of Bern